A product-regulated fructose 2,6-bisphosphatase occurs in green leaves
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منابع مشابه
Interaction of Tl+ with product complexes of fructose-1,6-bisphosphatase.
Fructose-1,6-bisphosphatase requires divalent cations (Mg2+, Mn2+, or Zn2+) for catalysis, but a diverse set of monovalent cations (K+, Tl+, Rb+, or NH(4)(+)) will further enhance enzyme activity. Here, the interaction of Tl+ with fructose-1,6-bisphosphatase is explored under conditions that support catalysis. On the basis of initial velocity kinetics, Tl+ enhances catalysis by 20% with a K(a) ...
متن کاملEnzyme regulation in C4 photosynthesis. Purification and properties of thioredoxin-linked fructose bisphosphatase and sedoheptulose bisphosphatase from corn leaves.
Homogeneous preparations of thioredoxin-linked fructose-l,6-bisphosphatase and sedoheptulose-l,7-bisphosphatase were prepared from leaves of corn (Zea mays), a classical C4 plant. Fructose-1,6-bisphosphatase had a molecular weight of 184,000 and consisted of four apparently identical subunits of M, = 46,000. Sedoheptulose-1,7-bisphosphatase, by contrast, was composed of two identical subunits o...
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Two isoforms of fructose 1,6-bisphosphatase are present in the green alga Selenastrum minutum. The isoenzymes can be separated with ionexchange chromatography or acid precipitation. The stability of the two isoenzymes differ largely. The acid insoluble enzyme exhibits properties similar to that of the enzyme from the chloroplasts of higher plants, i.e. an alkaline pH optima in the absence of re...
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A cDNA encoding 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase was isolated from a Spinacia oleracea leaf library and used to express a recombinant enzyme in Escherichia coli and Spodoptera frugiperda cells. The insoluble protein expressed in E. coli was purified and used to raise antibodies. Western blot analysis of a protein extract from spinach leaf showed a single band of 90.8 kDa. So...
متن کاملCrystallographic data for chicken liver fructose bisphosphatase.
Large, single crystals of fructose bisphosphatase have been obtained under a variety of conditions. Preliminary crystallographic analysis reveals that the space group is R3, the cell dimensions on the hexagonal axes are a = b = 304 A and c = 80.4 A, and there is one tetramer per asymmetric unit.
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1983
ISSN: 0014-5793
DOI: 10.1016/0014-5793(83)81057-6